The Spinsolve 80 ULTRA is ideal to study biochemical processes like fermentations. The high sensitivity of the 80 MHz model makes it possible to detect metabolites produced at sub millimolar concentrations in just minutes. In this particular example, solvent suppression was combined with carbon decoupling to eliminate the carbon satellites of the NMR signals of ethanol produced during the fermentation process.
The Spinsolve 80 ULTRA device was used in combination with our reaction monitoring software package to follow the course of a reaction on-line and also to determine its stereochemical outcome. As an example, the well-known Horner-Wadsworth-Emmons reaction (HWE reaction) was carried out.The HWE reaction was essentially developed to form the E-diastereoisomer as the reaction product in favour of the corresponding