The two detectors measure sample and reference respectively and simultaneously for optimizing measurement accuracy. They provide excellent performance for measurements in the range of 190 to 1100nm. They are suitable for pharmaceutical, biochemical and clinical lab applications as well as routine applications such as quantitative analysis, kinetics, wavelength scan, multiple components and DNA/protein.
Specifications |
Double Beam Optical System with Automatic 8-Cell Changer - Model 3200 |
Wavelength Range |
190nm to 1100nm |
Working Mode |
Stand alone and PC controlled with window based application software UV/\/IS Analyst |
Spectral Band Width |
Variable 0.5, 1, 2, 4 nm |
Monochromator |
Double beam hollow graphic matings 1200 lines/mm |
Wavelength Display |
0.1nm |
Wavelength Setting |
0.1nm |
Wavelength Accuracy |
± 0.1 nm @656.1nm D2 |
|
± 0.3nm (190 to 1100nm) |
Wavelength Repeatability |
<0.1nm |
Stray Light |
0.02°%@ 340nm for NaN02 |
|
0.25% @ 198nm for Kcl |
Optional Accessory |
Specular Reflection Accessory, Integrating Sphere 60mm / 100 mm
Automatic 8 Cell changer, Rectangular cell holder for variable path length, Large sample compartment having stable and reproducible mounting of accessories for easy use of different cells., Peltier Solid Sample holder etc.
|
Photometric Range (Approx.) |
0-400%T Absorbance -4 to 4 |
Photometric Accuracy |
± 0.002 Abs(0.5) |
|
± 0.004 Abs(1.0) |
|
± 0.006 Abs(2.0) |
Photometric Reproducibility |
0.001 Abs(0.5 Abs) |
|
0.001 Abs(1.0 Abs) |
|
0.003 Abs(2.0 Abs) |
Baseline Stability |
< 0.0002 Abs/H @ 500 nm |
Baseline Flatness |
± 0.0005 Abs |
Noise Level |
0.000016 Abs RMS @ 500 nm |
Scan Speed |
1-6000nm per Minute |
Light Source |
Tungsten and Deuterium Lamp, pre-aligned |
Output |
USB Port and Parallel Port (Printer) |
DNA/Protein Measurement |
Included |
Dimension (W x D x H) |
600 x 450 x 200 nm |
Weight |
22kg |
Memory |
USB Memory Devices (standard accessory) |
Wavelength Slew Rate |
6500 nm/min. |
Lamp Interchange Wavelength |
Automatic interchange linked to wavelength. The interchange wavelength can be set freely. |
Detector |
Silicon Photodiode (Dual) |
The PC Models come standard with windows based application sofMare UV/Vis Analyst |
UV-Vis Spectroscopy (or Spectrophotometry) is a quantitative technique used to measure how much a chemical substance absorbs light. This is done by measuring the intensity of light that passes through optical components with respect to the intensity of light through a reference sample or blank.
There are four basic components to a simple single beam UV/Vis spectrophotometer; a light source, a monochromator, a sample, and a detector.
Molecules having non-bonding electrons can absorb the energy in the form of UV or visible light to excite these electrons to higher molecular orbitals. ... Ultraviolet-Visible Spectroscopy is absorption spectroscopy in the UV and visible portion of the electromagnetic spectrum.
In general, no -- at least from naturally occurring physical processes. Any form of radiation -- including visible light or radio waves -- could potentially be dangerous if highly concentrated into a narrow beam (that is the principle of lasers) of very high power.
UV / Vis spectrophotometer measures the absorbance of a light when it passes through a sample. The light absorbed is proportional to the quantity of a chemical in the sample.