Spectrophotometry is one of the most useful methods of quantitative analysis in various fields such as Agriculture, Soil, Environmental, Food, Metal, Mining, Petrochemical, Clinical, Pharmaceutical.A spectrophotometer is the device used to measure the intensity of electromagnetic energy at each wavelength of light in a specified region.
It mainly consists of a light source, a way to focus light onto the sample, a method to collect the light from the sample, a monochromator to separate the light into its component wavelengths and a detector to measure the intensity of light at each wavelength.
At Labindia, we manufacture state-of-the-art Spectrophotometers ranging from fully automatic ones to UV-VIS Spectrophotometers. The spectrometer is a highly flexible, versatile and high-quality instrument that is carefully crafted for the modern laboratory.
Model |
Specifications |
Optical system |
Double beam, Double Monochromator, Czerny Turner |
Bandwidth |
0.1/0.2/0.5/1.0/2.0/4.0/5.0 nm |
Wavelength range |
190-900nm |
Wavelength accuracy |
±0.1nm (D2656.1nm),±0.3nm all range |
Wavelength repeatability |
≤0.02nm |
Wavelength display |
0.1nm |
Resolution |
0.025nm |
Wavelength scan rate |
4000 to 0.5 nm/min |
Wavelength slew rate |
14500 nm/min |
Photometric accuracy |
±0.002 A @ 0.0-0.5 A ,+ 0.004 A @ 0.5-1 A, ±0.3%T @ 0-100 %T |
Photometric repeatability |
< 0> |
Baseline flatness |
±0.0008 A |
Baseline stability |
±0.0004Abs (200-860nm) |
Noise level |
< 0> |
Photometric range |
- 5.0 to +5.53 Abs |
Stray light |
< 0> |
Diffraction Grating |
1800 Lines/nm |
Detector |
PMT |
Light source |
D2/WI |
Optional Accessory |
Specular Reflection Accessory, Integrating Sphere 60mm / 100mm Automatic 8 Cell Changer, long path cell holder, Peltier Solid Sample holder etc. |
Interface |
LAN, USB, RS-232 |
Power supply |
160W |
Dimensions |
660 (W) x 570 (D) x 270 (H) mm |
Weight |
32 kg |
UV-Vis Spectroscopy (or Spectrophotometry) is a quantitative technique used to measure how much a chemical substance absorbs light. This is done by measuring the intensity of light that passes through optical components with respect to the intensity of light through a reference sample or blank.
There are four basic components to a simple single beam UV/Vis spectrophotometer; a light source, a monochromator, a sample, and a detector.
Molecules having non-bonding electrons can absorb the energy in the form of UV or visible light to excite these electrons to higher molecular orbitals. ... Ultraviolet-Visible Spectroscopy is absorption spectroscopy in the UV and visible portion of the electromagnetic spectrum.
In general, no -- at least from naturally occurring physical processes. Any form of radiation -- including visible light or radio waves -- could potentially be dangerous if highly concentrated into a narrow beam (that is the principle of lasers) of very high power.
UV / Vis spectrophotometer measures the absorbance of a light when it passes through a sample. The light absorbed is proportional to the quantity of a chemical in the sample.